TB-500
Thymosin Beta-4 fragment · TB4-Frag
A synthetic thymosin beta-4 peptide studied for cell migration and tissue repair in animal models.
Class
Synthetic fragment of thymosin beta-4 (Tβ4)
Half-life
Several hours (longer than most repair peptides)
Evidence
Animal models
Animal models
Evidence comes primarily from rodent or other animal studies. Translation to humans is unproven.
Overview
TB-500 is a synthetic peptide corresponding to an active region of thymosin beta-4, a naturally occurring protein involved in cell migration and actin regulation. Where BPC-157 is framed around angiogenesis, TB-500's signature mechanism is promoting the migration of repair cells into damaged tissue.
Thymosin beta-4 itself has been studied in humans for dry eye and cardiac repair. TB-500 (the fragment sold for recovery) has animal evidence for wound healing, cardiac tissue repair after injury, and flexibility/recovery in performance contexts — much of the early interest came from veterinary use in racehorses.
Like BPC-157, its human evidence base for musculoskeletal recovery is anecdotal, and the fragment is not identical to the full protein studied in clinical trials. It is WADA-prohibited and not approved for human use.
How it works
- Binds and sequesters G-actin, regulating actin polymerisation to enable cell migration.
- Promotes migration of endothelial and repair cells into injured tissue.
- Upregulates angiogenesis and has anti-inflammatory signalling.
- Longer tissue residence than smaller repair peptides, allowing less frequent dosing.
What the research shows
Researched effects
- Accelerated wound healing and reduced scarring in animal models
- Cardiac tissue repair after induced injury in rodents
- Improved recovery and flexibility in veterinary (equine) use
- Anti-inflammatory effects in various injury models
Limitations & what it won't do
- Human musculoskeletal evidence is anecdotal; the fragment differs from clinically studied full-length Tβ4.
- Optimal human dosing is not established.
- WADA-prohibited.
- Long-term safety in humans is uncharacterised; angiogenic activity carries the same theoretical tumour concern.
Dosing protocols
These ranges reflect published trials and community protocols. They are reference information, not a prescription. Doses are per injection unless noted.
Loading and maintenance (community)
2–2.5 mg loading; 2–2.5 mg maintenanceThe longer half-life is why TB-500 uses twice-weekly loading rather than daily dosing. Often paired with BPC-157.
Reconstitution & measuring your dose
Handling
- Typical vial sizes
- 2 · 5 · 10 mg
- Suggested BAC water
- 2 mL
- Storage
- Lyophilised powder refrigerated at 2–8 °C, protected from light.
- After reconstitution
- Reconstituted: approximately 30 days refrigerated.
Why 2 mL? A 10 mg vial in 2 mL yields 5 mg/mL — a 2 mg dose lands on 40 units, 2.5 mg on 50 units of a U-100 syringe.
Dose calculator
Draw to
80units
= 0.8 mL · 25 mcg per unit
Concentration
2500 mcg/mL
Doses per vial
2
Side effects
Common
- Transient fatigue or head rush after dosing
- Injection-site irritation
- Temporary lightheadedness
Serious / rare
- Unknown — no controlled human safety data
- Theoretical tumour concern via angiogenesis
Contraindications & interactions
Do not use if you have
- Active cancer or current oncology treatment
Promotes angiogenesis and cell migration; contraindicated during active malignancy.
- Pregnant, breastfeeding, or trying to conceive
No pregnancy safety data.
Use caution if you have
- Past cancer diagnosis (in remission)
Obtain oncology clearance given the angiogenic and pro-migratory mechanism.
What to monitor
- Injury response and function
- New or unusual symptoms given the absence of human safety data
Commonly combined with
References
- Goldstein AL et al. — Thymosin beta4: a multi-functional regenerative peptideExpert Opinion on Biological Therapy 2012;12:37-51
- Crockford D et al. — Thymosin beta4 in dermal and corneal wound healingAnnals NY Acad Sci 2010;1194:179-189
Research & educational information only — not medical advice.
You must be 18 or older to use this site. The peptides described are presented as research chemicals intended for research purposes only. Most are not approved by the FDA for human use. Dosing ranges reflect what has appeared in the scientific literature or community protocols and are not prescriptions. Nothing here replaces evaluation by a licensed physician who knows your full medical history.
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